Survival of the Virus of Poliomyelitis for Eight Years in Glycerol

1. An instance of successful inoculation of poliomyelitis virus after preservation for 8 years in 50 per cent glycerol is reported. 2. The virulence of the material injected remained essentially unchanged during this period. 3. The fact that poliomyelitis virus will survive in glycerol for so great a period may be taken as further indication of the improbability of streptococci as the inciting organisms. 4. Poliomyelitis virus would seem to vary in its resistance to glycerolation. 5. The remarkable persistence of active virus outside of the body may have a bearing on the epidemiology of poliomyelitis.